Journal: Nature Communications
Article Title: Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections
doi: 10.1038/s41467-021-27949-3
Figure Lengend Snippet: MACV GP1-Fc immobilized onto anti-human Fc biosensor tips and incubated with ( a ) sTfR1 or ( b ) buffer alone, ( c ) sTfR1 in complex with Fab ch128.1/IgG1 or ( d ) Fab ch128.1/IgG1 alone, or ( e ) sTfR1 in complex with Tf or ( f ) Tf alone. The arrows indicate the time points at which the indicated proteins were added and the dissociation step. The experiment was performed twice with representative data from one of the experiments shown. Seconds (s). Source data are included in the Source Data file.
Article Snippet: DNA encoding human sTfR1 (GenBank: NM_003234.3 residues 121–760) was cloned into the pHLsec expression vector downstream of the pHLsec secretion signal. sTfR1 was produced using linear polyethylenimine (PEI) to transfect HEK 293 S GnTI −/− cells (ATCC ® CRL-3022TM) grown in suspension culture and maintained in FreeStyle 293 Expression Medium (Cat. No. 12338018) supplemented with 2% Ultralow IgG FBS (Cat. No. 16250078) and penicillin/streptomycin (Thermo Fisher Scientific).
Techniques: Incubation